APC11, Mouse anti-Human, Clone: CRQ1.1, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABE1789100UG

Catalog No. MABE1789100

Description

Anti-APC11, clone CRQ1.1, Cat. No. MABE1789, is a highly specific mouse monoclonal antibody that targets Anaphase-promoting complex subunit 11 and has been tested in Western Blotting.

Anaphase-promoting complex subunit 11 (UniProt: Q9NYG5; also known as APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein) is encoded by the ANAPC11 (also known as HSPC214) gene (Gene ID: 51529) in human. APC11 is one of the components of the anaphase-promoting complex (APC) that belongs to the RING-box family of proteins. APC is a large E3 ubiquitin ligase that responsible for targeting proteins for degradation during mitosis. The APC is assembled from 13 different proteins where in APC2 binds to APC11 forming the catalytic core that recruits the E2 ubiquitin conjugating enzyme. APC11 has with multiple zinc -binding sites with a RING-type zinc-finger domain (aa 34-77). It is expressed at high levels in the skeletal muscle and heart and in moderate levels in brain, kidney, and liver. Two isoforms of APC11 have been described that are produced by alternative splicing. (Ref.: Schreiber, A et al., (2011). Nature. 470(7333); 227-32).

Specifications

• Antigen: APC11
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: ANAPC11;HSPC214
• Immunogen: A linear peptide corresponding to 9 amino acids from the C-terminal region of human Anaphase-promoting complex subunit 11 (APC11).
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone CRQ1.1 specifically detects human Anaphase-promoting complex subunit 11 (APC11). It targets an epitope with in 9 amino acids from the C-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG2a κ

• Applications: Western Blot
• Clone: CRQ1.1
• Dilution: Western Blotting Analysis: A representative lot detected APC11 in Western Blotting applications (Sedgwick, G.G., et. al. (2013). EMBO J. 32(2):303-14).Western Blotting Analysis: A representative lot detected APC11 in APC11-Flag expressed in HeLa cells following tetracycline treatment.
• Gene Alias: Anaphase-promoting complex subunit 11;Cyclosome subunit 11;Hepatocellular carcinoma-associated RING finger protein
• Host Species: Mouse
• Molecular Weight of Antigen: 9.84 kDa calculated.
• Quantity: 100 μg
• Research Discipline: Epigenetics & Nuclear Function
• Gene ID (Entrez): NP_001002244.1
• Target Species: Human
• Form: Purified