Invitrogen™ APIP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529269

Catalog No. PIPA529269

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (94%), Zebrafish (82%), Xenopus laevis (82%), Chicken (87%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf 1 (for apoptosis protease-activating factor 1). Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), which leads to Caspase-9 activation. Activated Caspase-9 in turn cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, Cho et al (4) have identified a new Apaf 1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Specifications

• Antigen: APIP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: APIP
• Gene Alias: APAF1 interacting protein; APAF1-interacting protein; APIP; APIP2; CG129; CGI29; CGI-29; dJ179L10.2; hAPIP; Methylthioribulose-1-phosphate dehydratase; Mmrp19; monocyte macrophage 19; monocyte/macrophage protein 19; MTRu-1-P dehydratase; probable methylthioribulose-1-phosphate dehydratase; RGD1564562
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51074
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.01 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q96GX9
• Gene Symbols: APIP
• Immunogen: Full length human APIP Recombinant protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG