ApoA1 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P33150UG	Invitrogen™ 500P33150UG	50 μg
500P331100	Invitrogen™ 500P331100UG	100 μg
500P3311MG	Invitrogen™ 500P3311MG	1 mg

Description

AA Sequence of recombinant protein: MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ApoA-I. Anti-Human ApoA-I-specific antibody was purified by affinity chromatography employing an immobilized Human ApoA-I matrix. Sandwich ELISA: To detect Human ApoA-I by sandwich ELISA (using 100 μL/well), a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ApoA-I (500-P331BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human ApoA-I. Western Blot: To detect Human ApoA-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human ApoA-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

Specifications

• Antigen: ApoA1
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P02647
• Gene ID (Entrez): 335
• Immunogen: E.coli-derived, 28.2 kDa Recombinant Human ApoA-I.
• Purification Method: Antigen affinity chromatography
• Gene: APOA1

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 1-Sep; 2-Sep; Alp-1; Apo a; APO A1; apo A-1; APO I; apo(a); APOA1; Apoa-1; ApoAI; Apo-AI; apoA-I; Apolipoprotein; Apolipoprotein A1; apolipoprotein A-1; Apolipoprotein AI; apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A-I(1-242); Brp-14; Ltw-1; Lvtw-1; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; prepro-apolipoprotein-AI; ProapoA-I; Proapolipoprotein A-I; Sep-1; Sep2; Sep-2; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
• Gene Symbols: APOA1
• Primary or Secondary: Primary
• Content And Storage: -20°C