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Invitrogen™ ApoA1 Recombinant Rabbit Monoclonal Antibody (19H20L19)

Catalog No. PI701239
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100 μg
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Catalog No. Quantity
PI701239 100 μg
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Catalog No. PI701239 Supplier Invitrogen™ Supplier No. 701239
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Description

Rabbit Recombinant Monoclonal Antibody

This antibody is predicted to react with mouse and non-human primate based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. By sandwich ELISA, 701239 can be used as a coating antibody with each of the following mouse monoclonal detection antibodies: Product # MA5-14668, MA5-14670, or MA5-14732, to generate a matched pair. With each of these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, is detected. 701239 can be used to capture Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with 701239, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
TRUSTED_SUSTAINABILITY

Specifications

Antigen ApoA1
Applications ELISA, Western Blot, Immunocytochemistry
Classification Recombinant Monoclonal
Clone 19H20L19
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide
Gene APOA1
Gene Accession No. P02647, P04639
Gene Alias 1-Sep; 2-Sep; Alp-1; Apo a; APO A1; apo A-1; APO I; apo(a); APOA1; Apoa-1; ApoAI; Apo-AI; apoA-I; Apolipoprotein; Apolipoprotein A1; apolipoprotein A-1; Apolipoprotein AI; apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A-I(1-242); Brp-14; Ltw-1; Lvtw-1; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; prepro-apolipoprotein-AI; ProapoA-I; Proapolipoprotein A-I; Sep-1; Sep2; Sep-2; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
Gene Symbols APOA1
Host Species Rabbit
Immunogen Peptide corresponding to amino acids 127-142 of human Apolipoprotein A1.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 25081, 335
Target Species Human, Rat
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
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