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Apolipoprotein E3/ApoE3 Mouse anti-Human, Clone: 960318, R&D Systems™

Mouse Monoclonal Antibody

$113.85 - $339.00

Specifications

Antigen Apolipoprotein E3/ApoE3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Format Purified
Clone 960318
Applications Immunohistochemistry
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
MAB41443SP View Documents R&D Systems
MAB41443SP
25 ug Each for $113.85
Add to cart
 
MAB41443100 View Documents R&D Systems
MAB41443100
100 ug Each for $339.00
Add to cart
 
Description

Description

ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages, and non-neuronal cells in the CNS. ApoE-containing particles transport triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism (1-4). Mature human ApoE is a 37kDa glycoprotein that consists of an N-terminal domain composed of four bundled α-helices, plus a hinge region and an extended α-helical C-terminal domain (2, 5). Its amphipathic nature and flexible structure enables it to adopt dramatically different conformations upon lipid association (2). ApoE is monomeric in lipid particles, although it forms oligomers when lipid-free (6). ApoE3 is the most abundant of the three common alleles in human; ApoE2 and ApoE4 differ by single aa substitutions (1). Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. LDL receptor family proteins preferentially bind and internalize the lipid-bound form of ApoE with the exception of VLDLR which also efficiently internalizes lipid-free ApoE (7, 8). Lipoprotein uptake is facilitated by the initial binding of ApoE to cell surface heparan sulfate proteoglycans (HSPG) (9). Receptor/HSPG binding and lipid interactions primarily involve the N- and C-terminal regions of ApoE, respectively (2). Recycled lipid-free ApoE is formed into HDL particles through interactions with the lipid transporter ABCA1 (10). High cellular sterol content activates the nuclear hormone receptor LXR which promotes increased ApoE synthesis and increased sterol efflux, while low sterol content induces LDL R expression with increased sterol uptake and decreased ApoE production (11). ApoE3 dampens the TNF-α induced inflammatory response in vascular endothelial cells (12). In the CNS, ApoE blocks production of the amyloid Aβ peptide by inhibiting the γ-secretase cleavage of APP (13). It also complexes with Aβ and promotes Aβ internalization via LRP2 (14, 15).
Specifications

Specifications

Apolipoprotein E3/ApoE3
Purified
Immunohistochemistry
Mouse
Protein A or G purified from hybridoma culture supernatant
RUO
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
960318
Unconjugated
IgG1
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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