Invitrogen™ Apolipoprotein E4 Human ELISA Kit

Description

Sandwich ELISA Kit

The Human Apolipoprotein E4 (Hu ApoE4) ELISA quantitates Hu ApoE4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoE4. Principle of the method The Human ApoE4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13.2.

Specifications

• Accession Number: P02649
• Assay Range: 0.41 ng/mL to 100 ng/mL
• Assay Sensitivity: 0.4 ng/mL
• Calibration Range: 0.4 ng/mL to 100 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: Biotin
• Product Type: ELISA Kit
• Protein Family: GCPR
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human ApoE4. Other species not determined.
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 348
• Gene Symbol: AD2,APO-E,ApoE4,LDLCQ5,LPG
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Stop Solution, SAV-HRP, Assay Diluent Concentrate, Biotinylated Detection Antibody, Wash Buffer, Pre-coated 96 well plates, Adhesive Plate Covers, Chromogen, Standard
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Cardiovascular Research
• Sample Volume: Plasma, 1 μ;L; Serum, 1 μ;L; Supernatant, 100 μ;L
• Storage Requirements: 2°C to 8°C
• Synonym: AD2, APO-E, LDLCQ5, LPG
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 4 hr. 45 min.