AmpErase™ TaqMan™ Fast Universal PCR Master Mix 2X without AmpErase™ UNG Non-distribution product as customer accommodation.

Single-tube format for qPCR amplification and detection

Manufacturer: Applied Biosystems 4367846

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Catalog No. 43-678-46

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Required Accessories : Template and primers

    Specifications

    Detection Method Primer-Probe Detection
    Dye Type ROX (Pre-mixed)
    For Use With (Application) Fast Real-Time PCR, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling
    Includes TaqMan Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains one 50mL tube sufficient for 5000 20-μL PCR reactions.
    No. of Tests 5000.0
    Sample Type DNA (Genomic), cDNA
    Shelf Life Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
    Storage Requirements Store at 2-8°C

    Delivers results in 40 minutes for 40 cycles of PCR in a 20µL reaction volume using the Applied Biosystems™ 7900HT and Fast 7500 Fast Real-Time PCR Systems. Optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX™ dye serves as a passive internal reference to normalize non-PCR–related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

    • Convenience: Everything needed for TAqMan-based qPCR amplification and detection in a single-tube format, excluding the template and primers, for superior real-time qPCR
    • Compatibility: TAqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG is compatible with TAqMan Gene Expression assays

    For use with 7900HT Fast System, 7500 Fast System, StepOnePlus, Fast ModeStepOne, and Fast Mode

    Fast Real-Time PCR, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling

  • Description & Specifications

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