Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Description

Requires

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

• Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
• The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
• A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
• Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
• TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
• Compatible with TaqMan Gene Expression assays
• Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Concentration: 2X
• Content And Storage: TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 2 packs of 2 tubes of 1.25 mL each sufficient for 500 20 μL PCR reactions. Store at 2-8°C
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: AmpliTaq DNA Polymerase
• Reaction Speed: Fast
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: AmpErase™, TaqMan™
• Product Type: Fast Universal PCR Master Mix
• Quantity: 500 reactions
• Sufficient For: 500 Reactions
• For Use With (Application): Gene Expression, Genotyping, miRNA analysis
• Fidelity (vs. Taq): 2 X
• No. of Reactions: 500 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 20 μL

For Research Use Only. Not for use in diagnostic procedures.