Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Supplier:  Applied Biosystems™ 4398894

Catalog No. 43-988-94

Includes: AmpliTaq Gold 360 DNA Polymerase is supplied at 5U/μL with 10X AmpliTaq Gold 360 PCR Buffer, 25mM M2Cl₂, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq Gold 360 Buffer, 25mM MgCl₂, and 360 GC Enhancer is supplied with each 250U of enzyme. Storage Buffer Enzyme is supplied in 20mM Tris, pH 9.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% (w/v) Tween 20, 50% (v/v) glycerol. Source: Recombinant, modified form of theThermus aquaticusDNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

Description

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with AmpliTaq Gold 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. This polymerase efficiently amplifies targets present at low copy number, even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection and amplification of targets from degraded DNA samples. AmpliTaq Gold 360 DNA Polymerase delivers 360° coverage for a full range of targets.

AmpliTaq Gold 360 DNA Polymerase is purified by a proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.

Features

• Optimized for the broadest range of targets
• High sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with 360 GC Enhancer
• High-quality sequencing data
• Efficiently and reproducibly amplifies sequences up to 5 Kb

Optimized for easy and challenging targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (see figure).

Optimized for hot-start PCR

AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA Polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.

When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA Polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers.

Notes

• See figure below for a demonstration of robust PCR amplification of long human and plasmid DNA.
• See user's manual or package insert for limited label license and trademark information.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Content And Storage: • AmpliTaq Gold 360 DNA Polymerase (5 U/μL), 12 x 50 μL
  • 10X AmpliTaq Gold 360 Buffer, 12 x 1.5 mL
  • 25 mM MgCl₂, 12 x 1.5 mL
  • 360 GC Enhancer, 12 x 1.5 mL
  
  Store at -15°C to -25°C.
• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Green Features: Sustainable packaging
• Quantity: 3000 Units
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 2400 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Format: Tube
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase
• Shipping Condition: Room Temp or Wet Ice
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.