Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

Contains everything required for successful PCR amplification in a convenient package—all components are premixed and premeasured

Includes: AmpliTaq Gold 360 Master Mix is supplied at 2X concentration and the 360 GC Enhancer. Typically, 1.5mL of the 360 GC Enhancer is supplied with each 5mL of master Mix.. Source: Recombinant, modified form of theThermus aquaticusDNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

Catalog Number	Mfr. No.	Quantity
43-988-81	Applied Biosystems™ 4398881	5 mL
43-988-76	Applied Biosystems™ 4398876	1 mL
43-988-86	Applied Biosystems™ 4398886	50 mL
43-989-01	Applied Biosystems™ 4398901	10 x 5 mL

Description

AmpliTaq Gold 360 Master Mix was designed for 360° coverage of a full range of targets. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers. Designed for convenience, the AmpliTaq Gold 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase but it also contains the world-class 360 GC Enhancer which can be added optionally for high GC–content templates. With the wide template coverage, optimization of PCR reaction conditions, is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix.

• Optimized for broadest range of targets from everyday to challenging
• Unmatched sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Achieves highest-quality sequencing data
• Easy-to-use, premixed master mix
• Less waste, sustainable packaging

Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, Methylation-Sensitive Mobility Shift Assay, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Concentration: 2X
• Fidelity (vs. Taq): 1 X
• GC-Rich PCR Performance: High
• Overhang: 3'-A
• Reaction Format: SuperMix or Master Mix
• Size (Final Product): 5 kb or less
• Exonuclease Activity: 5' - 3'
• Product Line: AmpliTaq Gold™
• For Use With (Application): PCR Amplification

• Detection Method: Primer-Probe
• Format: SuperMix or Master Mix
• Hot Start: Built-In Hot Start
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Reaction Speed: Standard
• Starting Material: DNA
• Green Features: Sustainable packaging
• Product Type: PCR Master Mix

For Research Use Only. Not for use in diagnostic procedures.