Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Includes: AmpliTaq Gold 360 Master Mix is supplied at 2X concentration and the 360 GC Enhancer. Typically, 1.5mL of the 360 GC Enhancer is supplied with each 5mL of master Mix.. Source: Recombinant, modified form of theThermus aquaticusDNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

Catalog Number	Mfr. No.	Quantity
43-988-76	Applied Biosystems™ 4398876	1 mL
43-988-81	Applied Biosystems™ 4398881	5 mL
43-989-01	Applied Biosystems™ 4398901	10 x 5 mL
43-988-86	Applied Biosystems™ 4398886	50 mL

Description

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package with all components premixed and premeasured. Designed for convenience, this master mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase, but it also contains the world-class 360 GC Enhancer, which can be added for high GC-content templates. With the wide template coverage, optimization of PCR reaction conditions is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix.

Features

• Optimized for a broad range of targets
• High sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Helps achieves high-quality sequencing data
• Easy-to-use, premixed master mix

Optimized for easy and challenging targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions. Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as a high-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets.

Optimized for automated, hot-start PCR

AmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold 360 DNA Polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. The extreme specificity allows easier multiplexing and allelic discrimination.

Amplify low-copy amplicons and long targets

AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection and amplification of targets from degraded DNA samples. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences.

Notes

• AmpliTaq Gold 360 PCR Master Mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Format: Tube
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: PCR Master Mix
• For Use With (Application): Cloning, Hot-start PCR
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Green Features: Sustainable packaging
• Shipping Condition: Room Temp or Wet Ice
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.