High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor Non-distribution product as customer accommodation.

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2µg total RNA

Manufacturer: Applied Biosystems 4374966

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Catalog No. 43-749-66

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    For Use With (Application) cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
    Format Separate Components
    Includes 10X RT Buffer (1mL); 10X RT Random Primers (1mL); 25X dNTP Mix (100mM, 0.2mL); MultiScribe Reverse Transcriptase (0.2mL at 50U/μL); RNase Inhibitor (2 × 0.1mL at 20U/μL)
    Quantity 200 reactions
    Sample Type RNA
    Storage Requirements Store between -15deg. to -25deg.C
    Temperature (English) Operating 98.6deg.F
    Temperature (Metric) Operating 37deg.C

    The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2µg of total RNA to single-stranded cDNA in a single 20µL reaction. This kit also includes two tubes of RNase inhibitor (100µL each). Reactions can be scaled up to 100µL to generate 10µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

    • Linear target amplification for real-time PCR
    • Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
    • Ten-fold greater dynamic range than other kits
    • Optimal reaction temperature: 37°C
    • Final product size: 7kb or less

    Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

    PCR & Real-Time PCR, Reverse Transcription

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