MeltDoctor™ HRM Master Mix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Formulated for superior HRM performance across a wide range of genomic targets

Manufacturer: Applied Biosystems 4415440

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Catalog No. 44-154-40

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Required Accessories : Template and primers
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    For Use With (Application) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12K Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
    Includes 2X mixture of AmpliTaq Gold 360 DNA Polymerase, MeltDoctor HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components
    Quantity 5mL

    MeltDoctor HRM Master Mix contains all components (except template and primers) for High Resolution Melting analysis, a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. No additional mixing required prior to use.

    • Low background fluorescence
    • High brightness in the presence of double-stranded DNA
    • Minimal temperature shift of DNA melting due to dye binding
    • Thermal stability to tolerate PCR cycling conditions
    • No inhibition of polymerase activity, resulting in high PCR efficiency
    • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

    • Perform Mutation Scanning HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis. As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High-Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE). Specifically, the advantages of HRM for mutation scanning include: ·Low reagent consumption, with little waste: HRM requires only a 20µL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
    • Simple, fast workflow: no additional instrumentation is required after PCR amplification. A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
    • Fast optimization: unlike DHPLC, thermal optimization is not required
    • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction Quality Components
    • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
    • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing nonspecific product formation and enabling reactions to be set up at room temperature
    • MeltDoctor HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

    Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

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