Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

Provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis.

Supplier:  Applied Biosystems™ 4425557

Catalog No. 44-255-57

Includes: AmpliTaq Gold™ 360 DNA Polymerase, AmpliTaq Gold 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, MeltDoctor HRM Dye (20X)

Description

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

This kit includes:

• AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets
• GeneAmp™ dNTP Blend
• MeltDoctorHRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Content And Storage: This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.
  
  Store at -15° to -25°
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System
• Label or Dye: High Resolution Melting (HRM), SYTO 9
• Product Type: Hrm Reagent Kit
• For Use With (Application): Real Time PCR (qPCR)

• Detection Method: HRM
• PCR Method: qPCR
• Technique: HRM (High Resolution Melting) Analysis, Mutation Scanning
• Genotyping Target: SNPs (Unknown or Numerous), Epigenetic Targets
• Product Line: MeltDoctor™
• Quantity: 1 kit
• Sample Type: DNA (Genomic), cDNA

For Research Use Only. Not for use in diagnostic procedures.