Applied Biosystems™ Path-ID™ Multiplex One-Step Kit

Description

Includes

1.375mL of 2X Multiplex RT-PCR Buffer, 280μL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript, 1.75mL of Nuclease-free water

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).

It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan™ primer/probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with ArrayScript™ reverse transcriptase and AmpliTaq Gold™ DNA Polymerase, and a buffer mix.

Multiplex One-Step Kit is optimized for amplification of up to four targets simultaneously using your RNA samples and TaqMan™ primer/probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. Kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold™ DNA Polymerase, and a buffer mix.

Applications:

One-Step qRT-PCR, PCR & Real-Time PCR, Real Time PCR (qPCR)

Order Info

Built-In Hot Start. Guaranteed minimum shelf life is 60 days.

Specifications

• Content And Storage: • 1.375 mL of 2X Multiplex RT-PCR Buffer
  • 280 μL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript
  • 1.75 mL of Nuclease-free water
  • Store the kit at -20°C; the Nuclease-free water may be stored at -20°C, 4°C, or room temperature
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: Primer-probe
• Form: Liquid
• Format: Tube
• PCR Method: 1-step RT-qPCR, Multiplex qPCR
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: Path-ID™
• Product Type: One-Step RT-PCR Kit
• Quantity: 100 reactions
• Reverse Transcriptase: ArrayScript™
• Sufficient For: 100 Reactions
• For Use With (Application): Pathogen Detection, Virus Detection
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Sample Type: Total RNA, Viral RNA, mRNA

For Research Use Only. Not for use in diagnostic procedures.