Applied Biosystems™ SYBR™ Green Universal Master Mix

Description

Includes

2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability
• Premixed components stored at 2° to 8°C significantly reduce assay setup time

Maximum Flexibility and Convenience:

• Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
• SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
• The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
• Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
• SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
• Store at -20°C

Order Info

Shipping Condition: Wet Ice

Specifications

• Concentration: 2X
• Content And Storage: The 1 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 200 reactions based on a 50 μl reaction volume.
  
  Store at -20°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
  
  
• Detection Method: SYBR
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
• Label or Dye: SYBR Green
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: SYBR™
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 1 x 5 mL
• Shipping Condition: Wet Ice
• Sufficient For: 200 Reactions of 50 μL
• For Use With (Application): Gene Expression
• No. of Reactions: 200 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 1 x 5 mL

For Research Use Only. Not for use in diagnostic procedures.