Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.

Catalog Number	Mfr. No.	Quantity
43-444-63	Applied Biosystems™ 4344463	1 x 1 mL
43-091-55	Applied Biosystems™ 4309155	1 x 5 mL
43-643-44	Applied Biosystems™ 4364344	2 x 5 mL
43-643-46	Applied Biosystems™ 4364346	5 x 5 mL
43-127-04	Applied Biosystems™ 4312704	10 x 5 mL
43-349-73	Applied Biosystems™ 4334973	1 x 50 mL

Description

Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability
• Premixed components stored at 2° to 8°C significantly reduce assay setup time

Maximum Flexibility and Convenience:

• Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
• SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
• The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
• Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
• SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
• Store at -20°C

Order Info

Shipping Condition: Wet Ice

Specifications

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase
• Label or Dye: SYBR Green
• Product Line: SYBR™
• For Use With (Application): Gene Expression

• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Real Time PCR SYBR Master Mix
• Concentration: 2X

For Research Use Only. Not for use in diagnostic procedures.