Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Description

Requires

Template and primers

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis, pathogen detection and viral load quantification,co-amplification of two targets, as well as specific detection of gene family members

TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and Uracil-DNA Glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX™ dye for superb precision on Applied Biosystems™ real-time PCR instruments.

• Provides precise and reliable real-time qPCR quantification over nine logs of linear dynamic range
• TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target
• Extended benchtop stability provides consistent results for automated liquid-handling systems
• This makes it the choice for high-throughput sample processing overnight or over the weekend
• Validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications
• Applications include rare transcript detection, gene expression analysis, pathogen detection and viral load quantification, co-amplification of two targets, as well as specific detection of gene family members
• Store at 2°C to 8°C

TaqMan Gene Expression Master Mix contains:

• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets
• A blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination
• A passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments

Order Info

Shipping Condition: Wet ice

Specifications

• Content And Storage: 1 bottle (50 mL) 2X TaqMan™ Gene Expression Master Mix sufficient for 2000 reactions. Store at 2°C to 8°C.
  
  
  TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.
• Detection Method: Primer-probe
• Form: Liquid
• PCR Method: qPCR
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: TaqMan™
• Purity or Quality Grade: UP (Ultra Pure)
• Quantity: 1 x 50 mL
• Shipping Condition: Wet Ice
• For Use With (Application): Gene Expression
• No. of Reactions: 2000 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 1 x 50 mL

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.