Applied Biosystems™ TaqMan™ Reagents Starter Kit

TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.

Supplier:  Applied Biosystems™ 4352405

Catalog No. 43-524-05

Includes: TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG, Eukaryotic 18S rRNA TaqMan™ Assay (with two unlabeled PCR primers and a labeled TaqMan™ probe), plus a sample of human Raji cDNA

Description

Gene-specific, ready to use TaqMan™ Gene Expression Assays and Universal Master Mix provide you with a fast, simple way for measuring changes in gene expression.

• Simple procedure gives you quick results
• Hot-Start DNA Polymerase enzyme system minimizes nonspecific product amplification for reliable results
• Used With: 7500 Real-Time PCR System with Dell™ Notebook, 7500 Real-Time PCR System with Dell™ Tower, 7900HT Fast Real-Time PCR System with 384-well Block Module, 7900HT Fast Real-Time PCR System with 384-well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-well Block Module, 7900HT System Automation Accessory Upgrade, 7900HT System Standard 384-well Block Upgrade Kit, 7900HT System Standard 96-well Block Upgrade Kit, ABI PRISM™ 7000 Sequence Detection System (Notebook)

Ready-to-Use Gene Expression Solution:

• The TaqMan™ Reagents Starter Kit contains a sample of TaqMan™ Universal PCR Master Mix, and TaqMan™ Gene-Expression Assay (Eukaryotic 18S rRNA), plus a sample of human Raji cDNA - everything you need for 18S gene-expression analysis
• Simply combine these reagents according to the instructions to generate reproducible and quantitative gene-expression data

A Complete, Integrated Gene-Expression System:

• Eliminate time-consuming master mix formulation and assay optimization with the TaqMan™ Gene-Expression Assays and the TaqMan™ Universal PCR Master Mix, by using universal cycling conditions and pre-optimized reagents

Order Info

Shipping Conditions: Dry Ice

Specifications

• Content And Storage: This kit contains TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG, Eukaryotic 18S rRNA TaqMan™ Assay (with two unlabeled PCR primers and a labeled TaqMan™ probe), plus a sample of human Raji cDNA.
  
  Store the PCR mix at 2° to 8°C.
  Store the rest of the kit contents at -15° to -25°C.
• PCR Method: qPCR
• Reaction Speed: Standard
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: PCR Reagents
• Target: 18S rRNA
• For Use With (Application): Gene Expression
• Sample Type: DNA (Genomic), cDNA

• Detection Method: Primer-probe
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7000 System, 7500 System, AB 7900 HT 384-Well System
• Product Line: TaqMan™
• Shipping Condition: Dry Ice
• Target Specificity: Target-Specific
• Hot Start: Built-In Hot Start

For Research Use Only. Not for use in diagnostic procedures.