Invitrogen™ Aquaporin 2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA577841

Catalog No. PIPA577841

Description

0.5 μg/mL of PA5-77841 was sufficient for detection of aquaporin 2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. Detects approximately 28.8kDa. May detect larger glycosylated bands approximately 35-50kDa.

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking.

Specifications

• Antigen: Aquaporin 2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Aqp2
• Gene Alias: ADH water channel; AQP 2; AQP CD; Aqp2; AQP-2; AQPCD; AQP-CD; aquaporin 2; aquaporin 2 (collecting duct); Aquaporin CD; Aquaporin2; aquaporin-2; Aquaporin-CD; Aquaporine 2; collecting duct water channel protein; cph; jpk; MGC34501; Water channel protein for renal collecting duct; water-channel aquaporin 2; WCH CD; WCHCD; WCH-CD
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 11827, 25386, 359
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P34080, P41181, P56402
• Gene Symbols: Aqp2
• Immunogen: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG