Aquaporin-4, Mouse anti-Rat, Human, Mouse, Clone: mECD, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABN247125UG

Catalog No. MABN247125

Description

Anti-Aquaporin-4, clone mECD, Cat. No. MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation.

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NMO) from multiple sclerosis. Clone mECD is shown to induce clustering and internalization of Aquporin-4 and cause its redistribution, which is also observed with NMO-IgG. Exposure to mECD antibody is also shown to cause coaggregation of astrocytic CD28B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl.Acad. Sci. USA 114(21); 5491-5496).

Specifications

• Antigen: Aquaporin-4
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
• Gene Symbols: AQP4
• Immunogen: His-tagged full-length human recombinant Aquaporin-4.
• Quantity: 25 μg
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_001641
• Target Species: Rat, Human, Mouse
• Form: Purified

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Clone: mECD
• Dilution: Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
• Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG1 κ