Invitrogen™ ARC Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA519934

Catalog No. PIPA519934

Description

In Western blot applications, this antibody detects a band at ~25kDa. A suggested positive control is Hela cell lysate. PA5-19934 can be used with blocking peptide PEP-0059.

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms. Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. ARC inhibits extrinsic apoptotic pathways two different ways. The first is by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. The second way is by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, which in turn limits the amount of soluble CASP8 avaliable for DISC-mediated activation. ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX. This results in BAX inactivation and prevents mitochondrial dysfunction and release of pro-apoptotic factors. Mutations affecting the gene can results in myoclonus, familiam cortical.

Specifications

• Antigen: ARC
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOL3
• Gene Alias: activity regulated cytoskeletal-associated protein; activity regulated cytoskeleton associated protein; activity-regulated cytoskeleton-associated protein; Activity-regulated gene 3.1 protein; activity-regulated gene 3.1 protein homolog; Apoptosis repressor with CARD; ARC; arc {ECO:0000312; ARC/ARG3.1; Arc3.1; Arg3.1; B430311C09Rik; C86064; FCM; hArc; KIAA0278; mArc; Muscle-enriched cytoplasmic protein; MYC; MYP; Nol3; NOP; Nop 30; Nop30; Nucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); nucleolar protein of 30 kDa; rg3.1; RGD:62037}
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 78688, 85383, 8996
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O60936, Q62881, Q9D1X0
• Gene Symbols: NOL3
• Immunogen: A peptide corresponding to amino acids 2 to 18 of human origin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG