Molecular Probes™ ARES™ Alexa Fluor™ 488 DNA Labeling Kit 

ARES™ Alexa Fluor™ 488 DNA Labeling Kit

Manufacturer:  Molecular Probes™ A21665

Catalog No. A21665

Description

The ARES™ Alexa Fluor™ DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor™ dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor™ 488 dye. The labeled probes can be used for fluorescence in situ hybridization (FISH) and microarray techniques. We offer ARES™ Alexa Fluor™ DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5µg DNA each.

ARES™ Alexa Fluor™ Labeling Kit Specifications:

Dye (Ex/Em): Alexa Fluor™ 488 (492/520 nm)

Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides

Typically produces one dye per 12–20 bases

Optimal for FISH and microarrays

More Uniform Labeling With ARES™ Labeling Kits
ARES™ Alexa Fluor™ Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in our FISH Tag™ DNA and FISH Tag™ RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Specifications

• Description: ARES™ Alexa Fluor™ 488 DNA Labeling Kit, Fluorescent Detection Method, Alexa Fluor™ 488 Dye, DNA (General), cDNA Labeling Target, Store at –5 to –30°C, Used For Labeling Chemistry, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis
• Dye Type: Alexa Fluor™ 488
• For Use With (Application): DNA & RNA purification & analysis, cell analysis, labeling chemistry, labeling amines, amine-reactive fluorophores, nucleic acid chemical labeling, nucleic acid labeling & oligo synthesis, DNA labeling, gene expression analysis & genotyping, microarray analysis, expression array labeling
• Includes: 50μL 5-(3-aminoallyl)-dUTP (component A), 10 vials reactive dye (component B), 200μL Dimethylsulfoxide (DMSO) or dimethylformamide (DMF) (component C), 84mg Sodium bicarbonate (MW = 84.0) (component D), 10mL nuclease-free water (component E)
• Quantity: 1 Kit(s)
• Target: DNA (general), cDNA
• Formula Weight: 884.91

• Detection Method: Fluorescent
• Final Product Type: Probes (Labeled DNA), cDNA (Labeled)
• Formulation: Liquid
• Product Type: ARES™ Alexa Fluor™ 488 DNA Labeling Kit
• Storage Requirements: –5 to –30°C
• Molecular Formula: C39H44F4N4O11S2

For Research Use Only. Not for use in diagnostic procedures.