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Arginase 1 Rat anti-Human, Mouse, eFluor® 450, Clone: A1exF5, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  eBioscience 48369780

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Catalog No. 48-369-780


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Description

Description

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set. Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative for Pacific Blue™. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both, human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
Specifications

Specifications

Arginase 1
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P05089, Q61176
AI; ARG1; Liver Arginase; Type 1 Arginase; PGIF
Rat
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
11846, 383
Flow Cytometry
A1exF5
eFluor™ 450
ARG1
Liquid
Arg1
E.coli-derived recombit mouse Arginase 1
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
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