Invitrogen™ Arginase 1 Polyclonal Antibody

Catalog No.	Quantity
PIPA532267	500 μL

Catalog No. PIPA532267 Supplier Invitrogen™ Supplier No. PA532267

Description

Rabbit Polyclonal Antibody

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is hepatocellular carcinoma.

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Specifications

• Antigen: Arginase 1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 0.046 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene: ARG1
• Gene Accession No.: P05089, P07824, Q61176
• Gene Alias: AI; A-I; AI type I arginase; AI256583; Arg1; Arg-1; Arginase; arginase 1; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase1; arginase-1; HGNC:663; Liver Arginase; Liver-type arginase; PGIF; similar to arginase, type I; Type 1 Arginase; type I arginase
• Gene Symbols: ARG1
• Host Species: Rabbit
• Immunogen: Synthetic peptide derived from the N-terminus of human arginase-1 protein.
• Purification Method: Antigen affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11846, 29221, 383
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov