Argos Technologies™ CELLine Bioreactor Flasks

Specialized cell culture flask for production of monoclonal antibodies or expressed proteins from suspension cells

Catalog Number	Capacity (Metric)	Culture Area
03-391-207	1000 mL	1 x 109 cells / cell compartment
03-391-208	350 mL	2 x 108 cells / cell compartment

Description

Unlike traditional culture flasks, CELLine separates the cells from the media and serum, thereby dramatically reducing purification and concentration costs. A 10,000 MWCO cellulose acetate membrane between the chambers allows nutrients to move into the cells and waste products to move out.

• High yield antibody production with no need for special equipment
• Flasks can be used continuously under aseptic conditions
• Bottom silicone membrane allows easy CO₂ and O₂ exchange for improved cell growth and viability
• Viable in vitro alternative to using mouse ascites
• Cells and cellular-secreted products with a MWCO over 10kD are retained between two non-cytotoxic membranes
• Separate ports give access to either media or cell cultivation chamber, allowing easy change of expended medium without interference with cell growth, function, or gas exchange
• Polystyrene housing with molded silicone gas transfer bottom
• Stackable; save incubator space
• Gamma irradiation sterilized and pressure tested

Model CL1000

• Ideal for monoclonal antibody production up to 5mg/mL, recombinant protein expression, and concentrated cell densities of 108 cells/mL
• Produces up to 80mg antibody per month
• Allows production of up to 1 × 109 cells per compartment
• 15 mL cultivation chamber is ideal for small production runs
• 1000mL media compartment has vertical and horizontal volume markings from 100 to 1000 mL

Model CL350

• Designed for suspension cells
• Produces up to 27 mg antibody per month
• Allows production of up to 2 × 10⁸ cells per compartment
• 5mL cultivation chamber is ideal for all tissues where cell density is important
• 350mL media compartment has vertical and horizontal volume markings from 50 to 350mL

Specifications

• Material: Polystyrene
• For Use With (Application): For use with CHO, BHK, HEP2 or any other adherent cell line, for monoclonal Antibody production up to 5mg/mL
• Shape: Rectangular