Invitrogen™ ARHGEF7 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA588071

Catalog No. PIPA588071

Description

Immunogen sequence: MNSAEQTVTW LITLGVLESP KKTISDPEGF LQASLKDGVV LCRLLERLLP GTIEKTFDAN DLYQGQNFNK VLSSLVTLNK VTADIGLGSD SVCARPSSHR IKSFDSLGSQ SLHTRTSKLF QGQYRSLDMT DNSNNQLVVR AKFNFQQTNE DELSFSKGDV IHVTRVEEGG WWEGTLNGRT GWFPSNYVRE VKASEKPVSP KSGTLKSPPK GFDTTAINKS YYNVVLQNIL ETENEYSKEL QTVLSTYLRP LQTSEKLSSA; Positive Samples: SW620, Jurkat, COS-1, COS-7, MCF7, K-562, Mouse lung, Mouse heart; Cellular Location: Cell junction, Cell projection, Cytoplasm, cell cortex, focal adhesion, lamellipodium, ruffle.

The serine/threonine kinase, p21 activated kinase (PAK), is a downstream effector of the small GTPases Cdc42 and Rac. PAK associates with Nck, the p85 and p110 subunits of PI3-kinase and PIX (PAK-interacting exchange factor) in a focal complex. The binding of PIX is necessary for the localization and activation of PAK in the Cdc42 to Rac signaling pathway, and this binding occurs through the high affinity of the N-terminal SH3 domain of PIX for a conserved proline rich PAK sequence. PIX exists as two isoforms, alpha and beta, and both are highly expressed in heart, muscle and thymus tissues of human and rat. alphaPIX is phosphorylated via PDGF and EphB2 receptor signaling pathways or through association with PI3-kinase. The alphaPIX isoform predominantly acts as a guanine nucleotide exchange factor (GEF) on Rac, which may mediate lamellipodia formation.

Specifications

• Antigen: ARHGEF7
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Arhgef7
• Gene Alias: ARHGEF7; betaPix; Beta-Pix; betaPix-b; betaPix-c; Cool; COOL1; cool-1; KIAA0142; mKIAA0142; Nbla10314; P50; P50BP; p85; p85Cool1; p85SPR; PAK3; Pak3 binding protein; Pak3bp; PAK-interacting exchange factor beta; PIX; Pixb; PIXbeta; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor (GEF7); Rho guanine nucleotide exchange factor 7; SH3 domain-containing proline-rich protein
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54126, 8874
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.48 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q14155, Q9ES28
• Gene Symbols: Arhgef7
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_0011069841).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Non-human Primate
• Product Type: Antibody
• Isotype: IgG