ARK5 Polyclonal Antibody, Invitrogen™

Goat Polyclonal Antibody

Supplier:  Thermo Scientific PA518919

Catalog No. PIPA518919

Description

This antibody is predicted to react with rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.

NUAK1 (ARK5) is a serine/threonine kinase that plays a role in many cellular functions. The overexpression of this kinase has been associated with tumor invasion and metastasis (colorectal neoplasm). Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.

Specifications

• Antigen: ARK5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NUAK1
• Gene Alias: AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; AU014801; AW494241; B230104P22Rik; I79_017442; KIAA0537; NUAK family kinase 1; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; Nuak1; Omphalocele kinase 1; Omphk1; RGD1309956; ZNUAK family, SNF1-like kinase, 1
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 9891
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O60285
• Gene Symbols: NUAK1
• Immunogen: Synthetic peptide sequence (QNRPRPQYLKRYRNR) corresponding to the internal amino acids of NUAK1
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov