ARK5 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5106252

Catalog No. PIPA5106252

Description

Antibody detects endogenous levels of total NuaK1.

NUAK1 (ARK5) is a serine/threonine kinase that plays a role in many cellular functions. The overexpression of this kinase has been associated with tumor invasion and metastasis (colorectal neoplasm). Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.

Specifications

• Antigen: ARK5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NUAK1
• Gene Alias: AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; AU014801; AW494241; B230104P22Rik; I79_017442; KIAA0537; NUAK family kinase 1; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; Nuak1; Omphalocele kinase 1; Omphk1; RGD1309956; ZNUAK family, SNF1-like kinase, 1
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 299694, 77976, 9891
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O60285, Q641K5
• Gene Symbols: NUAK1
• Immunogen: A synthesized peptide derived from human NUAK1(Accession O60285), corresponding to amino acid residues D575-L625.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG