ARL3 Mouse anti-Human, Clone: OTI3E1, lyophilized, TrueMAB™

Mouse Monoclonal Antibody

Supplier:  OriGene CF803089

Catalog No. 50-166-6320

Description

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.

Specifications

• Antigen: ARL3
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: ARL3
• Gene Alias: ARFL3
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 403
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Western Blot
• Clone: OTI3E1
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene Accession No.: P36405
• Gene Symbols: ARL3
• Immunogen: Human recombit protein fragment corresponding to amino acids 2-182 of human ARL3 produced in E.coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1