Invitrogen™ ARL3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529971

Catalog No. PIPA529971

Description

Recommended positive controls: mouse brain. Predicted reactivity: Mouse (97%), Rat (96%), Zebrafish (95%), Pig (99%), Sheep (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.

Specifications

• Antigen: ARL3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Arl3
• Gene Alias: ADP ribosylation factor like GTPase 3; ADP-ribosylation factor like GTPase 3; ADP-ribosylation factor-like 3; ADP-ribosylation factor-like protein 3; ADP-ribosylation-like 3; ARD3; ARFL3; ARF-like 3; Arf-like protein 3; Arl3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 403, 56350
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.38 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P36405, Q9WUL7
• Gene Symbols: Arl3
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ARL3. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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