Invitrogen™ ASAH2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520575

Catalog No. PIPA520575

Description

A suggested positive control is 293 cell lysate. PA5-20575 can be used with blocking peptide PEP-0695.

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

Specifications

• Antigen: ASAH2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Asah2
• Gene Alias: acylsphingosine deacylase 2; AI585898; Asah2; BCDase; hCD; HNAC1; LCDase; mitochondrial ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; N-acylsphingosine amidohydrolase 2; NCDase; N-CDase; Neutral ceramidase; Neutral ceramidase soluble form; neutral/alkaline ceramidase; non-lysosomal ceramidase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 114104, 54447, 56624
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q91XT9, Q9JHE3, Q9NR71
• Gene Symbols: Asah2
• Immunogen: A 17 amino acid peptide near the carboxy terminus of the human ASAH2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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