Invitrogen™ ASIC1 Monoclonal Antibody (N271/44), PerCP

Catalog No.	Quantity
PIMA545594	100 μg

Catalog No. PIMA545594

Supplier: Invitrogen™ MA545594

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-45594 was sufficient for detection of ASIC1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 60kDa. This antibody was formerly sold as clone S271-44.

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.

Specifications

• Antigen: ASIC1
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: N271/44
• Concentration: 1 mg/mL
• Conjugate: PerCP
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene: ASIC1
• Gene Accession No.: P55926, P78348, Q6NXK8
• Gene Alias: Accn2; ACCN2 variant 3; acid sensing (proton gated) ion channel 1; acid sensing ion channel; acid sensing ion channel 1; acid sensing ion channel subunit 1; acid-sensing (proton-gated) ion channel 1; Acid-sensing ion channel; Acid-sensing ion channel 1; acid-sensing ion channel 1a protein; AI843610; Amiloride-sensitive cation channel 2, neuronal; Asic; ASIC1; ASIC1a; B530003N02Rik; BNAC2; brain sodium channel 2; Cation channel, amiloride-sensitive, neuronal, 2; degenerin 2; hBNaC2; proton gated cation channel ASIC1
• Gene Symbols: ASIC1
• Host Species: Mouse
• Immunogen: Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11419, 41, 79123
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1