ASIC1 Polyclonal Antibody, Invitrogen™

Guinea Pig Polyclonal Antibody

Supplier:  Thermo Scientific PA5111818

Catalog No. PIPA5111818

Description

Reconstitute antibody with 50 μL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Peptide sequence is: CQKEAKRSSA DKGVALSLDD Homology: Human amino acid residues identical; mouse- 19/20 amino acid residues identical.

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.

Specifications

• Antigen: ASIC1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ASIC1
• Gene Alias: ACCN2; ACCN2 variant 3; Acid-sensing ion channel; Acid-sensing ion channel 1; AI843610; Amiloride-sensitive cation channel 2, neuronal; Asic; Asic1; ASIC1a; B530003N02Rik; Bnac2; Brain sodium channel 2; hBNaC2
• Host Species: Guinea Pig
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11419, 41, 79123
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
• Concentration: 0.8 mg/mL
• Formulation: PBS with 1% BSA and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P55926, P78348, Q6NXK8
• Gene Symbols: ASIC1
• Immunogen: Peptide corresponding to amino acid residues 469-488 of rat ASIC1 (Intracellular, C-terminus)
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG