Aspartate Transaminase Polyclonal Antibody, Rockland™

Sheep Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200601144S

Catalog No. 50-199-1246

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart]. Cross reactivity against Aspartate Transaminase (AST) from other tissues and species may occur but have not been specifically determined.

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Specifications

• Antigen: Aspartate Transaminase
• Classification: Polyclonal
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P00503
• Gene Symbols: GOT1
• Immunogen: Anti-Aspartate Transaminase antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart.
• Regulatory Status: RUO
• Gene ID (Entrez): 396967
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized

• Applications: ELISA, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Gene: GOT1
• Gene Alias: AI789014; aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; Aspat; AST1; ASTQTL1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Gaspat; GIG18; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; Got1; Got-1; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A
• Host Species: Sheep
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Pig
• Product Type: Antibody