Tocris Bioscience™ Asunaprevir

Description

Potent hepatitis C virus (HCV) NS3 protease inihibitor

Asunaprevir is a potent HCV NS3 protease inhibitor (IC50 = 0.2-3.5 nM). Asunaprevir can be used to control CRIPSR-Cas9 gene editing via the small molecule-assisted shut-off (SMASh) technique, which involves fusion of Cas9 to a peptide comprising a protease domain and a degron tag. Asuprenavir blocks the protease-mediated removal of the degron tag leading to degradation of Cas9 through proteasome and autophagolysosome pathways. It can also be used as a resversible switch to control CAR (chimeric antigen receptor)-T cells, where the CAR has been modified by insertion of a hepatitis C virus nonstructural protein 3 protease (HCV-NS3) between the scFv and the hinge domains. Also inhibits SARS-CoV-2 Mpro activity (IC50 = 15-48.4 μM).

Specifications

• Chemical Name or Material: 1-[[N-tert-Butoxycarbonyl-L-tert-leucyl-trans-4-[(7-chloro-4-methoxyisoquinolin-1-yl)oxy]-L-prolyl]amino]-N-(cyclopropylsulfonyl)-(1 R,2 S)-2-vinylcyclopropane-1-carboxamide
• CAS: 630420-16-5
• Quantity: 50 mg
• Target: CRISPR Reagents
• Molecular Formula: C₃₅H₄₆ClN₅O₉S
• Purity: 0.98