Invitrogen™ Ataxin 1 Monoclonal Antibody (N65/37), FITC

Catalog No.	Quantity
PIMA545706	100 μg

Catalog No. PIMA545706 Supplier Invitrogen™ Supplier No. MA545706

Description

Mouse Monoclonal Antibody

Rat: 93% identity (15/16 amino acids identical). Human: 87% identity (14/16 amino acids identical). 1 μg/mL of MA5-45706 was sufficient for detection of Ataxin 1 in 20 μg of COS cells transiently transfected with Ataxin-1 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 85kDa. No cross-reactivity against phosphor S751-Ataxin-1. Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot. This antibody was formerly sold as clone S65-37.

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the 'pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 41-81 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for this gene.

Specifications

• Antigen: Ataxin 1
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: N65/37
• Concentration: 1 mg/mL
• Conjugate: FITC
• Formulation: PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
• Gene: ATXN1
• Gene Accession No.: P54253, P54254, Q63540
• Gene Alias: 2900016G23Rik; alternative ataxin1; ataxin 1; Ataxin1; ataxin-1; Atx1; Atx-1; Atx-1-PB; Atxn1; C85907; CG4547; CG4547-PB; D6S504E; dAtx-1; Dmel\CG4547; Dmel_CG4547; ENSMUSG00000074917; Gm10786; OTTHUMP00000016065; SCA1; spinocerebellar ataxia 1; spinocerebellar ataxia 1 homolog; spinocerebellar ataxia type 1 protein; Spinocerebellar ataxia type 1 protein homolog
• Gene Symbols: ATXN1
• Host Species: Mouse
• Immunogen: Synthetic peptide amino acids 746-761 (RKRRWSAPETRKLEKS) of mouse ataxin-1.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20238, 25049, 6310
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov