Invitrogen™ ATF1 Monoclonal Antibody (7F8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549203

Catalog No. PIMA549203

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1. Positive Control - WB: human HepG2 whole cell, human Hela whole cell, human SW620 whole cell. IHC: human lung cancer tissue, human rectal cancer tissue, human rectal cancer tissue. ICC/IF: A431 cell. Flow: K562 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.

Specifications

• Antigen: ATF1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: P18846
• Gene Symbols: ATF1
• Immunogen: E.coli-derived human ATF1 recombinant protein (Position: M1-V271).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 7F8
• Conjugate: Unconjugated
• Gene: ATF1
• Gene Alias: AATase 1; Activating transcription factor 1; alcohol O-acetyltransferase; Alcohol O-acetyltransferase 1; ATF1; Atf-1; cAMP-dependent transcription factor ATF-1; cyclic AMP-dependent transcription factor ATF-1; EWS-ATF1; FUS/ATF-1; Protein TREB36; TCR-ATF1; TREB36; Unknown (protein for MGC:64099); wu:fc92c07; YOR377W; zgc:64099
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 466
• Content And Storage: -20°C
• Form: Lyophilized