Invitrogen™ ATF4 Monoclonal Antibody ([N360A/24)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527721

Catalog No. PIMA527721

Description

1 μg/mL of MA5-27721 was sufficient for detection of ATF4 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 60kDa. Does not cross-react with ATF5. This antibody was formerly sold as clone S360A-24.

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Specifications

• Antigen: ATF4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.4
• Gene Accession No.: P18848, Q06507, Q9ES19
• Gene Symbols: Atf4
• Immunogen: Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
• Clone: [N360A/24
• Conjugate: Unconjugated
• Gene: Atf4
• Gene Alias: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); activating transcription factor 4a; activating transcription factor 4b1 (tax-responsive enhancer element B67); activating transcription factor ATF-4; ATF 4; atf4; Atf-4; atf4 {ECO:0000312; ATF4 protein; atf4a; atf4b1; C/ATF; C/EBP-related ATF; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB2; CREB-2; Cyclic AMP-dependent transcription factor ATF-4; cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; hypothetical protein LOC509107; OTTHUMP00000199130; rATF-4; RGD:621863}; tax-re; TaxREB67; TaxREB67 homolog; tax-responsive enhancer element B67; tax-responsive enhancer element-binding protein 67; Tax-responsive enhancer element-binding protein 67 homolog; TXREB; wu:fj01c08; wu:fk30b04; zgc:85951
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 11911, 468, 79255
• Content And Storage: -20°C
• Form: Liquid