Invitrogen™ ATG12-ATG5 Recombinant Rabbit Monoclonal Antibody (20H20L20)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 702433

Catalog No. 70-243-3

Description

This antibody is predicted to react with Monkey, Pig, Cat and Mouse. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

Specifications

• Antigen: ATG12-ATG5
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O94817, Q2TBJ5, Q3MQ06, Q99J83, Q9CQY1, Q9H1Y0
• Gene Symbols: ATG12, ATG5
• Immunogen: Peptides corresponding to Human ATG12 (aa 43-57, 122-133).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: 20H20L20
• Conjugate: Unconjugated
• Gene: ATG12
• Gene Alias: 2010107M05Rik; 3110067M24Rik; 4931423H11Rik; A330058M13Rik; Apg12; Apg12 (autophagy, yeast) homolog; APG12L; APG12-like; APG5; Apg5l; APG5-LIKE; apoptosis-specific protein; ASP; Atg12; ATG12 autophagy related 12 homolog; Atg12l; Atg5; ATG5 autophagy related 5 homolog; Atg5l; Autophagy protein 5; autophagy related 12; autophagy related 5; autophagy-related 12; autophagy-related 5; autophagy-related protein 12; AW319544; C88337; FBR93; HAPG12; hAPG5; Paddy; Ubiquitin-like protein ATG12
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 11793, 361321, 365601, 67526, 9140, 9474
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid