Invitrogen™ ATG12 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532180

Catalog No. PIPA532180

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, Rat brain, ATG12 shRNA-transfected 293T. Predicted reactivity: Pig (81%), Chimpanzee (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.

Specifications

• Antigen: ATG12
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ATG12
• Gene Alias: 4931423H11Rik; A330058M13Rik; APG 12; APG12; Apg12 (autophagy, yeast) homolog; APG12L; APG12-like; ATG12; ATG12 autophagy related 12 homolog; Atg12l; Atg12p; Atg12-PB; Autophagy protein 12-like; autophagy related 12; autophagy related protein 12; autophagy-related 12; autophagy-related protein 12; Autophagy-specific gene 12; CG10861; CG10861-PB; Dmel\CG10861; Dmel_CG10861; FBR93; HAPG12; IP05205p; IP05405p; OTTHUMP00000159129; ubiquitin-like protein ATG12; YBR1506; YBR217W
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 361321, 67526, 9140
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1.36 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O94817, Q2TBJ5, Q9CQY1
• Gene Symbols: ATG12
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG