Invitrogen™ ATG13 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA587483

Catalog No. PIPA587483

Description

Positive Samples: HeLa, A-549, NCI-H460, SH-SY5Y, Rat brain, Rat heart; Cellular Location: Cytoplasm, Preautophagosomal structure, cytosol Immunogen sequence: ADLAYPVVFA AGLNATHPHQ LMVPGKEGGV PLAPNQPVHG TQADQERLAT CTPSDRTHCA ATPSSSEDTE TVSNSSEGRA SPHDVLETIF VRKVGAFVNK PINQVTLTSL DIPFAMFAPK NLELEDTDPM VNPPDSPETE SPLQGSLHSD GSSGGSSGNT HDDFVMIDFK PAFSKDDILP MDLGTFYREF QNPPQLSSLS.

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.

Specifications

• Antigen: ATG13
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ATG13
• Gene Alias: 1110053A20Rik; Atg13; ATG13 autophagy related 13 homolog; autophagy related 13; autophagy-related protein 13; D2Ertd391e; FLJ20698; Harbi1; Harbi1l; harbinger transposase derived 1; KIAA0652; OTTHUMP00000233321; OTTHUMP00000233322; OTTHUMP00000233323; OTTHUMP00000233324; OTTHUMP00000233325; OTTHUMP00000233326; PARATARG8; RGD1310685
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 362164, 51897, 9776
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 0.68 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O75143, Q91YI1
• Gene Symbols: ATG13
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_0011361451).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG