Invitrogen™ ATG14 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5121113

Catalog No. PIPA5121113

Description

Positive test controls include: HeLa, HCT116, 293T, SH-SY5Y, Neuro-2a, Mouse testis, Rat testis. The target is usually found in the following locations: Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Peripheral membrane protein, Preautophagosomal structure membrane, autophagosome membrane. Immunogen sequence: SKQKFTRAVK KLNANILYLC FSQHVNLDQL QPLHTLRNLM YLVSPSSEHL GRSGPFEVRA DLEESMEFVD PGVAGESDES GDERVSDEET DLGTDWENLP S.

ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator). Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. Autophagy is induced as a cellular response to nutrient stress which includes the formation of autophagosomes, fusion of these with the lysosome and formation of the autophagolysosome. The Class III PI3-Kinase, Vps34 and its interacting partner Beclin 1 have been shown to form a complex which specifically includes ATG 14 (type 1) or UVRAG (type 2). Atg 14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.

Specifications

• Antigen: ATG14
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Atg14
• Gene Alias: 4832427M01; Atg14; ATG14 autophagy related 14 homolog; Atg14L; Atg14-like protein; autophagy related 14; autophagy-related protein 14-like protein; Bakor; Barkor; beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein; D14Ertd114e; D14Ertd436e; Kiaa0831; RGD1304610; VATG14 autophagy related 14 homolog
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100504663, 22863, 305831
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 0.89 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: D4A4K3, Q6ZNE5, Q8CDJ3
• Gene Symbols: Atg14
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-440 of human ATG14 (NP_055739.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG