Invitrogen™ ATG4B Recombinant Rabbit Monoclonal Antibody (JE59-97)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA550822

Catalog No. FEMA550822

Description

Positive control: 293T cell lysate, Jurkat cell lysate, PC-12 cell lysate, mouse heart tissue lysate, mouse pancreas tissue lysate. Predicted band size: 44 kDa Subcellular Location: Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion.

Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4bis a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.

Specifications

• Antigen: ATG4B
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: Q8BGE6, Q9Y4P1
• Gene Symbols: Atg4b
• Immunogen: Recombinant protein within Human ATG4B aa 221-320/393.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: JE59-97
• Conjugate: Unconjugated
• Gene: Atg4b
• Gene Alias: 2510009N07Rik; APG4 (ATG4) autophagy-related homolog B; APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B; Atg4b; atg4b protein; Atg4bl; AUT2B; Aut2B1; Aut2b2; AUTL1; AUT-like 1 cysteine; AUT-like 1 cysteine endopeptidase; AUT-like 1, cysteine endopeptidase; autophagin 1; autophagin-1; Autophagin-2B; autophagy related 4 homolog B; autophagy related 4B cysteine peptidase; autophagy related 4B, cysteine peptidase; autophagy-related 4B; autophagy-related cysteine endopeptidase 1; Autophagy-related cysteine endopeptidase 2B; autophagy-related protein 4 homolog B; AW048066; bAut2B; Cysteine protease ATG4B; cysteine protease involved in autophagy APG4-B; hAPG4B; KIAA0943
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 23192, 316640, 66615
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid