ATG5 Rabbit anti-Human, Mouse, Rat, Polyclonal, Proteintech

Catalog No.	Quantity
50-172-6087	150 μL

Catalog No. 50-172-6087 Supplier Proteintech Group Inc Supplier No. 101812AP150UL

Description

Rabbit Polyclonal Antibody

This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Specifications

• Antigen: ATG5
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 0.27 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
• Gene: ATG5
• Gene Accession No.: Q3MQ06, Q99J83, Q9H1Y0
• Gene Alias: APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5
• Gene Symbols: Atg5
• Host Species: Rabbit
• Immunogen: ATG5 Fusion Protein Ag0214
• Purification Method: Antigen Affinity Chromatography
• Quantity: 150 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11793, 365601, 9474
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG