Invitrogen™ ATG8 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA126648

Catalog No. PIPA126648

Description

PA1-26648 detects a band of approximately 14 kDa. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast. Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies. Autophagic bodies form a double-membraned structure called the autophagosome, which subsequently fuses with the vacuole/lysosome. This process similar in mammals. Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively. Apg12 is synthesized in its mature form and seems to have one target, Apg5. Almost all Apg12 molecules are conjugated with Apg5.

Specifications

• Antigen: ATG8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ATG8
• Gene Alias: APG8; ATG8; AUT7; Autophagy-related protein 8; Autophagy-related ubiquitin-like modifier ATG8; CVT5; Cytoplasm to vacuole targeting protein 5; ubiquitin-like protein ATG8; YBL0732; YBL078C
• Host Species: Rabbit
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 852200
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 5 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: A6ZKM4
• Gene Symbols: ATG8
• Immunogen: Recombinant, full length S. cerevisiae protein.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Yeast
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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