Invitrogen™ ATG9A Monoclonal Antibody (14F2 8B1)

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen™ MA1149

Catalog No. PIMA1149

Description

MA1-149 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. MA1-149 has been successfully used in Western Blot, Immunofluorescence, and ELISA procedures. Western Blot analysis with MA1-149 shows the detection of a double band at ~85-95 kDa in human ATG9 overerexpression lysates. MA1-149 also detects additional unknown bands at ~40 and ~60 kDa. In Immunofluorescence applications, MA1-149 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.

Specifications

• Antigen: ATG9A
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: Q5FWU3, Q68FE2, Q7Z3C6
• Gene Symbols: ATG9A
• Immunogen: peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 14F2 8B1
• Conjugate: Unconjugated
• Gene: ATG9A
• Gene Alias: Apg9; APG9 autophagy 9-like 1; Apg911; APG9A; APG9L1; APG9-like 1; ATG 9A; Atg9; ATG9 autophagy related 9 homolog A; ATG9 autophagy related 9 homolog A (S. cerevisiae); Atg9a; Atg9l1; AU019532; autophagy 9-like 1 protein; autophagy protein 9; autophagy related 9A; autophagy-related 9A; autophagy-related 9-like 1; autophagy-related protein 9; autophagy-related protein 9A; FLJ22169; mATG9; MGD3208; RGD1310450; zgc:158700
• Host Species: Armenian Hamster
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 245860, 363254, 79065
• Content And Storage: -20°C
• Form: Liquid