Invitrogen™ ATP1A3 Monoclonal Antibody (XVIF9-G10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3915

Catalog No. MA3915

Description

MA3-915 detects sodium/potassium ATPase from human, monkey, bovine, sheep, canine, rabbit, guinea pig, mouse and rat tissue. This antibody is specific for the alpha-3 subunit. MA3-915 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. Immunohistochemical staining of sodium/potassium ATPase in rat retina with MA3-915 yields a pattern consistent with plasma membrane localization. The MA3-915 antigen is canine cardiac microsomes.

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

Specifications

• Antigen: ATP1A3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.05% sodium azide; pH 7.2
• Gene Accession No.: P06687, P13637, Q6PIC6
• Gene Symbols: ATP1A3
• Immunogen: Canine cardiac microsomes.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Sheep, Guinea Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: XVIF9-G10
• Conjugate: Unconjugated
• Gene: ATP1A3
• Gene Alias: AHC2; ATP1A1; Atp1a3; ATPa1a3; Atpa-2; ATPase Na+/K+ transporting subunit alpha 3; ATPase, Na+/K+ transporting, alpha 3 polypeptide; ATPase, Na+K+ transporting, alpha 3 subunit; CAPOS; DYT12; EC 3.6.3.9; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ ATPase 3; Na+/K+ -ATPase alpha 3 subunit; RDP; Sodium Potassium ATPase; Sodium pump 3; sodium pump subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain; sodium/potassium-transporting ATPase subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100349382, 100712985, 101103751, 232975, 24213, 282598, 478, 612463
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid