Invitrogen™ Aurora B Monoclonal Antibody (6G2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547462

Catalog No. PIMA547462

Description

Aurora B Monoclonal Antibody for Western Blot, ICC/IF, IHC

The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

Specifications

• Antigen: Aurora B
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 5mM sodium azide
• Gene Accession No.: Q96GD4
• Gene Symbols: AURKB
• Immunogen: Full length human recombinant aurora B protein expressed in and purified from E. Coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Canine, Horse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: 6G2
• Conjugate: Unconjugated
• Gene: AURKB
• Gene Alias: Aik2; AIM1; AIM-1; AIRK2; AL022959; ARK2; ARK-2; AurB; AURKB; aurkb-sv1; aurkb-sv2; aurora 1; aurora- and IPL1-like midbody-associated protein 1; aurora B; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora/IPL1-related kinase 2; aurora-1; aurora-B; aurora-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase; serine/threonine kinase 12; serine/threonine kinase 5; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; STK1; STK-1; Stk12; STK12; AIK2; AIM1; ARK2; IPL1; AIM-1; STK5
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 100073050, 479492, 9212
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid