Invitrogen™ Aurora B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA186828

Catalog No. PIPA186828

Description

Predicted to react with bovine, chimpanzee, dog, mouse, pig and rat based on sequence homology. In Western blot, this antibody detects a band at ~39kDa in HeLa cell lysate. Rabbit anti Human Aurora-B kinase antibody detects human Aurora-B kinase (AurB), a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC).

The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

Specifications

• Antigen: Aurora B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: AURKB
• Gene Alias: Aik2; AIM1; AIM-1; AIRK2; AL022959; ARK2; ARK-2; AurB; AURKB; aurkb-sv1; aurkb-sv2; aurora 1; aurora- and IPL1-like midbody-associated protein 1; aurora B; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora/IPL1-related kinase 2; aurora-1; aurora-B; aurora-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase; serine/threonine kinase 12; serine/threonine kinase 5; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; STK1; STK-1; Stk12; STK12; AIK2; AIM1; ARK2; IPL1; AIM-1; STK5
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 114592, 20877, 360192, 396955, 479492, 9212
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with <0.1% sodium azide; pH 7.2
• Gene Accession No.: O55099, O70126, Q7YRC6, Q96GD4, Q9N0X0
• Gene Symbols: AURKB
• Immunogen: Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Monkey, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov