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Invitrogen™ Avidin Polyclonal Antibody, HRP
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Catalog No. PA126844
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PA126844 1 mg
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Catalog No. PA126844 Supplier Invitrogen™ Supplier No. PA126844
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Description

Rabbit Polyclonal Antibody

PA1-26844 detects Avidin. PA1-26844 will not react with streptavidin. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Avidin
Applications Dot Blot, ELISA, Immunohistochemistry, Western Blot
Classification Polyclonal
Concentration 10 mg/mL
Conjugate HRP
Formulation PBS with 1% BSA and 0.01% gentamicin sulfate; pH 7.2
Gene AVD
Gene Accession No. P02701
Gene Alias AVD; avID; Avidin; unnamed protein product
Gene Symbols AVD
Host Species Rabbit
Immunogen Avidin collected from hen egg white.
Purification Method IgG fraction
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 396260
Target Species Chicken
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
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